AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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STUDIA CHEMIA - Ediţia nr.4 din 2010 | |||||||
Articol: |
EXPRESSION OF PHENYLALANINE AMMONIA-LYASES IN ESCHERICHIA COLI STRAINS. Autori: KLAUDIA KOVÁCS, ANDRÁS HOLCZINGER, BEÁTA VÉRTESSY, LÁSZLÓ POPPE. |
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Rezumat: Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) catalyzes the non-oxidative conversion of L-phenylalanine into (E)-cinnamate. PAL can be applied in organic synthesis, and can be considered also for enzyme supplementation cure for genetic disorder phenylketonuria. The aim of this study was to find optimal expression parameters of several previously cloned PAL’s (bacterial, plant and a chimera) in pBAD vectors for further functional characterization. Investigation of the expression level of PAL''''s in E. coli hosts with SDS PAGE analysis as well as activity assay of the recombinant PAL enzymes was performed. Keywords: phenylalanine ammonia-lyase, heterologous protein expression, biocatalysis, phenylketonuria |
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