Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI

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A simple and reliable thin layer chromatography (TLC) method for simultaneous separation of some carotenoids (β-carotene, lycopene, lutein, astaxanthin and zeaxanthin), retinoids (retinol, retinoic acid, 9-cis-retinal and all-trans-retinal) and tocopherols (α-tocopherol, γ-tocopherol and δ-tocopherol) was described. For a proper separation of all compounds three different mobile phases (Benzene: Petroleum Ether, 10:90, v/v; Methanol: Benzene: Ethyl acetate, 5:75:20, v/v/v; Benzene: Petroleum Ether, 90:10, v/v) were tested, using silica gel F₂₅₄ as stationary phase. The visualization was performed under UV light (254 and 365 nm). The presented method is simple and adequate for the separation and identification of these compounds from different matrices, such as pharmaceuticals and foods.

Keywords: carotenoids, retinoids, tocopherols, thin layer chromatography