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Analytical method and uncertainty estimation for the measurement of total phosphorus in aquatic media was performed in two different laboratories. Method performance criteria used for validation process were investigated on standard phosphorus samples: concentration linearity domain (by calibration curve), limit of detection (LOD), limit of quantitation (LOQ), precision, accuracy and robustness. In order to announce reliable results, uncertainties sources were identified, and different types of uncertainty were estimated. The validated method was further applied for total phosphorus determination from the sludge resulted at one municipal wastewaters treatment plant. Samples were collected from wastewater treatment sludge, before and after the dehydration process. The results show that the method is suitable and gives trustful results for phosphorus determination from wastewater sludge.

Keywords: wastewaters, sludge, total phosphorus, method validation, uncertainty estimation.