AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI
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Autori: ANDREEA-MELISA TRIPON, ADORJÁN CRISTEA, TIMEA KRISTEA, CĂLIN GABRIEL FLOARE, HORIA LEONARD BANCIU.
Published Online: 2021-06-30
Published Print: 2021-06-30
pp. 18-19
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ABSTRACT: Polyhydroxybutyrate (PHB) is a natural polyester which is produced under nitrogen and/or phosphorous limitation and excess of carbon (C) source. Under these conditions, the C source is converted to PHB and stored as intracellular C and energy reserves. PHB derivatives might be used as alternatives to synthetic plastics due to their biodegradability and biocompatibility. The aim of this study was to assess the ability of the extremely halotolerant bacterium Halomonas elongate DSM 2581T to synthesise PHB from two renewable C sources namely industrial (IM) and commercial molasses (CM). To induce PHB production, a liquid mineral medium with high salinity (8% w/v NaCl) was used. Three C sources where used separately in the experiments (final concentration of C-source 1% w/v) (D-glucose – as control, IM and CM) and 0.1% w/v yeast extract. Batch cultures were incubated at 37oC and 180 rpm. For cell count and PHB visualisation, DAPI and Nile Red staining where employed. 1H-NMR spectroscopy and crotonic acid assay were used for the chemical analysis of the PHB (Cristea et al., 2018). The highest PHB yield was obtained after 96 h of growth on D-glucose (2.61 g/L), followed by CM (2.63 g/L after 90 h) and IM (1.07 g/L after 48 h). The weight percentage (wt%) of PHB fraction was 85% when cultivation occurred on CM, 86% on D-glucose and 35% on IM. Our preliminary results have showed that this strain could use renewable C sources (like molasses) to produce PHB that may be further applied in sustainable circular economy strategy.
Key words: Halomonas elongate, high salinity, molasses, polyhydroxy-butyrate, renewable source.
