Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI

În partea de jos este prezentat rezumatul articolului selectat. Pentru revenire la cuprinsul ediţiei din care face parte acest articol, se accesează linkul din titlu. Pentru vizualizarea tuturor articolelor din arhivă la care este autor/coautor unul din autorii de mai jos, se accesează linkul din numele autorului.

 
       
         
    STUDIA BIOLOGIA - Ediţia nr.1 din 2019  
         
  Articol:   HALO-THERMOPHILIC PROTEASE FROM HALOCOCCUS AGARILYTICUS GUGFAWS-3 (MF425611).

Autori:  SANKET K. GAONKAR, IRENE FURTADO.
 
       
         
  Rezumat:  Halococcus agarilyticus GUGFAWS-3 is an archaeon isolated from a marine, white sponge (Haliclona sp.) inhabiting rocks in the intertidal region of Anjuna, Goa, India. Its cells have glycerol diether moieties and R-O-R and long isoprenoid chains, detectable in FTIR analysis, and have 99.1% 16S rRNA gene similarity to Halococcus agarilyticus 62ET of the family Halococcaceae (Gaonkar & Furtado, 2018). Uniquely, this organism simultaneously produces halo-extremozymes in 25 % NaCl, corresponding to 49.5 U mL−1 of protease and 3.67 U mL−1 of lipase. The halophilic-protease is constitutively produced at low levels regardless of the availability of substrates (Kalisz, 1998). The enzyme was partially purified by ethanol precipitation, dialysis and Sephadex G-200 gel filtration chromatography to 12.26 fold and is active over a broad range of temperatures (30-80°C); NaCl concentrations (0- 5 M) and pH (3-13), with optimum activity at 70°C, 3 M NaCl and pH 7. The enzyme is thermo-stable at 70°C for 120 h, retaining 40 % of its activity. Furthermore, it is stable in polar and non-polar organic solvents, detergents and hydrocarbons. Metal cations enhance its activity in the order of Ca2+> Ni2+> Fe3+> Co2+> Mg2+>Cu2+> Mn2+. Partially inhibited by β-mercaptoethanol, PMSF and EDTA, the enzyme is a cysteine, serine and metal dependent protease. We report here a neutral halo-thermophilic protease. This is the first time such an extremozyme is reported from strain GUGFAWS-3 and also for the genus Halococcus. Efforts to study its various biotechnological potentials will also be highlighted and discussed.

Keywords: haloarcheon, halo-extremozyme, halostable, marine Haliclona sp., protease.
 
         
     
         
         
      Revenire la pagina precedentă