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AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI În partea de jos este prezentat rezumatul articolului selectat. Pentru revenire la cuprinsul ediţiei din care face parte acest articol, se accesează linkul din titlu. Pentru vizualizarea tuturor articolelor din arhivă la care este autor/coautor unul din autorii de mai jos, se accesează linkul din numele autorului. |
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STUDIA PHYSICA - Ediţia nr.1 din 2016 | |||||||
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LIDOCAINE - HSA BINDING CHARACTERIZED BY FLUORESCENCE SPECTROSCOPY AND MOLECULAR DOCKING. Autori: SILVIA NEAMTU, LUIZA BUIMAGA-IARINCA, M. BOGDAN, IOAN TURCU. |
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Rezumat: Published Online: 2016-06-01 Published Print: 2016-06-30 VIEW PDF: LIDOCAINE - HSA BINDING CHARACTERIZED BY FLUORESCENCE SPECTROSCOPY AND MOLECULAR DOCKING Quenching fluorescence and molecular docking methods were used to evaluate changes in the local environment of intrinsic fluorophores of HSA in the presence of lidocaine and to calculate the binding parameters that characterize drug-protein interaction. We show that lidocaine induces significant fluorescence quenching of tryptophan and changes in conformation of IIA domain of HSA. The bimolecular quenching rate constant calculated using Stern-Volmer equation indicates a direct binding as the cause of fluorescence quenching. The protein-ligand association constant determined from Trp fluorescence quenching data showed a weak binding of lidocaine to HSA. The molecular docking calculations indicates three docking sites for lidocaine, in IIIA and IIA and IB domains of HSA, with preference for cavities located in IIIA. Keywords: fluorescence quenching spectroscopy; lidocaine-HSA interaction; molecular docking. |
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