AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
|
|||||||
The STUDIA UNIVERSITATIS BABEÅž-BOLYAI issue article summary The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name. |
|||||||
STUDIA PHYSICA - Issue no. SPECIAL ISSUE / 2001 | |||||||
Article: |
GAS-CHROMATOGRAPHY -MASS SPECTROMETRIC METHOD FOR LIVER FUNCTION TEST IN CHILDREN. Authors: M. CULEA, M. NANULESCU, MIRELA POPA, R. MIHALCA. |
||||||
Abstract: A gas chromatography-mass spectrometric (GC/MS) method for caffeine levels determination in blood to children suffering of different forms of liver diseases is described. Caffeine clearance is a novel approach for assessing hepatic microsomal function. 10 µg/ml 15N-theophylline has been used as internal standard. A Hewlett Packard GC/MS engine 5989B in the SIM mode was used equipped with a HP-5 capillary column 30mx 0.25mm diameter, 0,25µm film support, in the temperature program: 200-250oC with a rate of 10oC/min. The method was validated in the range 0-20µg/ml caffeine. A dose of 4mg/kg p.o. was used. Blood caffeine concentrations were measured before dose and timed intervals at 0, 30 min, 1, 3, 6, 9 and 12 h. Caffeine clearance, measured in patients with cirrhosis and chronic hepatitis, was reduced and half live time was increased in children with liver disease as compared with control. The decreased metabolism observed in patients with various forms of liver disease was correlated to the disease status. | |||||||