AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: F. NEKVAPIL, CS. MÜLLER MOLNÁR, S. TOMŠIĆ, S. CINTĂ PINZARU.
DOI: 10.24193/subbphys.2019.08
Published Online: 2019-12-30
Published Print: 2019-12-30
pp. 75-82
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Carotenoids present a group of tetraterpenoid biomolecules which are well known for their physiological benefits through their remarkable radical scavenging activity and reactive oxygen species quenching. However, little is known about role of carotenoids in relieving mechanical stress. Hence, in this study, we exposed sea urchin fertilized eggs to mechanical stress in form of centrifugation and exploited the advantage of Resonance Raman scattering to probe if the carotenoid profile or their distribution would change after application of the stress. Silver nanoparticles were used ro probe the Raman signal of carotenoids near the cell surface and attempt to achieve SERRS conditions (Surface-enhanced Resonance Raman scattering). We have found that carotenoid concentration notably increased on the cell surface after centrifugation, indicating that carotenoids were mobilized in response to mechanical stress.
Key words: Raman spectroscopy, mechanical stress, sea urchin eggs, carotenoids
