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    STUDIA GEOLOGIA - Issue no. 1 / 2007  
         
  Article:   EFFECT OF DDT ON THE DIET-TISSUE FRACTIONATION FACTORING IN VARIOUS TISSUES OF THE PERCH PERCA FLUVIATILIS.

Authors:  DAMIEN BANAS, HÉLÈNE ROCHE, YAN VOLLAIRE. 		 
       
         
  Abstract:  In ecotoxicological studies, stable isotopes (15N and 13C) are more and more used as a complementary method of dietary analysis, to describe trophic links and to assess food-web structure. Stable isotopes are regarded as indicators of organic matter sources able to describe trophic links and matter transfer along a food chain. These studies allow an accurate determination of the trophic level, based on the calculation of the diet-tissue fractionation factor. However the latter, determined for the whole organism or for particular tissues, may vary substantially in natura. Vanderklift and Ponsard’s (2003) analyses point to several important sources of variation in δ15N enrichment such as tissues, diet, taxon, main biochemical form of nitrogen excretion or nutritional status. The potential effect of pollutants on the diet-tissue fractionation factor is seldom taken into account. Therefore we raised perch, Perca fluviatilis in controlled conditions, and fed them for 6 weeks with commercial pellets contaminated with DDT, an ubiquist contaminant. DDT exhibite estrogenic activity stimulates production of liver microsomal enzymes that lead to hydroxylation of steroids in experimental animals which may have adversely affected reproductive success and development. In the present study, δ13C and δ15N were analysed in lipid-free tissues (liver, gills, white muscle), showing various metabolic activities.  
         
     
         
         
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