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Secondary metabolites were obtained from Chinese medicinal plant, Ligularia sibirica by using coupled hydrodistillation-extraction method. The essential oil was extracted by microwave assisted hydrodistillation, analysis of these volatile compounds was made with GC-MS. The ethanolic extract was re-extracted with chloroform; the identification of the pyrrolizidine alkaloids were made with thin layer chromatography. The main identified volatile compounds were sabinene, limonene, and terpinolene, and the alkaloids in the extract were most probably, tussilagine and iso-tussilagine.

Keywords: Ligularia sibirica, microwave assisted hydrodistillation, essential oils, pyrrolizidine alkaloids