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    STUDIA CHEMIA - Issue no. 4 / 2016  
         
  Article:   HPLC ANALYSIS, ANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF AN EXPERIMENTAL PLANT BASED GEL, FOR ENDODONTIC USAGE.

Authors:  .
 
       
         
  Abstract:  VIEW PDF: HPLC Analysis, Antimicrobial and Antifungal Activity of an Experimental Plant Based Gel, for Endodontic Usage

The aim of this study is to investigate the active compounds of an experimental gel and to evaluate its antimicrobial and antifungal effects. This gel consists of a mix extract of Arctium lappa L. root powder and Aloe vera (L.) Burm.f. gel and is used in endodontic procedures. With the help of HPLC/ESI( )-MS method, various active compounds were identified in the experimental extract. For the evaluation of the antimicrobial and antifungal activity, microorganisms commonly found in endodontic infections were used. The agar-diffusion method was performed for each plant formula and the microdilution method was used to obtain the minimum inhibitory concentration and also the minimum bactericidal concentration. Clear inhibition zones around discs indicated the presence of antimicrobial and antifungal activity for the disc diffusion assay. The minimum inhibitory and bactericidal concentration obtained were compared to the antibiotics or antifungals, specific for each microorganism.

Keywords: antimicrobial, antifungal, HPLC/ESI( )-MS, agar-diffusion, microdilution, Arctium lappa, Aloe vera

Published Online: 2016-12
 
         
     
         
         
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