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    STUDIA CHEMIA - Issue no. 4 / 2014  
         
  Article:   UPLC-QTOF-ESI(+) MS AND DIRECT MS INJECTION USED TO FINGERPRINT RESTING AND STIMULATED SALIVA PROFILES: PRELIMINARY RESULTS.

Authors:  MARIA CRISAN, CARMEN SOCACIU.
 
       
         
  Abstract:  A rapid and reliable profiling of resting and stimulated saliva by two advanced techniques, LC-QTOF-ESI ( ) MS and direct injection mass spectrometry (DIMS) was performed. Male and female healthy volunteers (n=12) were randomly selected, their resting, and stimulated saliva being collected, before and after chewing stimulation with parafin. Base peak chromatograms of saliva methanolic extracts (BPC) were recorded, the main peaks were identifed and the MS data (m/z values) were used to identify specific biomarkers. The biostatistic analysis made by Principal Component Analysis was applied to discriminate between samples’ profile. The comparative UPLC-QTOF-ESI( )MS fingerprints, before and after storage at -20ºC showed similar data with DIMS analysis, but the later one identified a larger range of molecules, without a preliminary separation by UPLC. Around 10 major biomarkers were identified, mainly phosspholipid derivatives, showing quantitative differences among the resting and stimulated saliva. Such preliminary results will be used for early diagnosis and monitoring therapy’s effects in dental pathology. 

Keywords: resting and stimulated saliva, metabolomics, UPLC-QTOF-MS, direct MS injection
 
         
     
         
         
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