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Sol-gel encapsulation of lipases proved to be a particularly easy and effective way to enhance the mechanical and catalytic properties of biocatalysts. The sol-gel encapsulated enzymes usually retain their selectivity whereas their heat stability or specific activity may be significantly improved. The aim of our work was to improve the immobilization of lipases in supported sol-gel systems. First, the binding properties of lipase AK on various solid supports were studied. Next, the immobilization properties of the best adsorbent-lipase combinations were tested in sol-gel encapsulation using tetraethoxy-silane/octyltriethoxy-silane/phenyltriethoxy-silane 1/0.7/0.3 silane precursor system.

Keywords: lipase, biocatalysis, adsorption, supported sol-gel immobilization