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    STUDIA CHEMIA - Issue no. 3 / 2020  
         
  Article:   DETERMINATION OF BIOGENIC AMINES BY USING AMPEROMETRIC BIOSENSORS BASED ON GRASS PEA AMINE OXIDASE AND OAT POLYAMINE OXIDASE.

Authors:  LAURA AQUILANTE, MIHAELA NISTOR, STEFANIA TAORMINA, MAREK ŠEBELA, IVO FRÉBORT, IONEL CĂTĂLIN POPESCU.
 
       
         
  Abstract:  
DOI: 10.24193/subbchem.2020.3.01

Published Online: 2020-09-30
Published Print: 2020-09-30
pp. 9-21
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ABSTRACT: Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by cross-linking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed.

Key words: Amine oxidase, polyamine oxidase, amperometric reagentless biosensors, biogenic amines, human urine
 
         
     
         
         
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