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The biosorption of phenol from aqueous solution on non-living mycelial pellets of Phanerochaete chrysosporium was studied using batch technique with respect to pH, initial concentration and biomass dosage. Ph. chrysosporium was grown in a liquid medium with a simple constitution. The phenol biosorption studies on fungal biomass was carried out at an initial pH of 5. Adsorption kinetics was characterized at an initial concentration of 12.5, 25 and 50 mg/L in a suspension concentration of 5.0 g/L. The sorption process followed the second-order kinetics. Phenol adsorption isotherms were determinated on fungal biomass at biomass concentration of 1.0 and 5.0 g/L and initial pH of 5. The adsorption equilibrium of phenol from aqueous solutions by mycelial pellets could be well described with Freundlich equation. The adsorption capacity of phenol and the Freundlich constant decreased with increasing biomass concentration.