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    STUDIA CHEMIA - Issue no. 2, Tom I / 2019  
         
  Article:   ISOLATION, PURIFICATION AND CHARACTERIZATION OF ASCORBATE OXIDASE AND PEROXIDASE FROM CUCURBITA PEPO MEDULLOSA.

Authors:  ALINA-MARIANA CRAINIC, AUGUSTIN C. MOȚ, RADU SILAGHI-DUMITRESCU. 		 
       
         
  Abstract:  Ascorbate oxidase is a well-known copper-containing enzyme however, its biological role and mechanism are still unclear. Reported here is a new protocol for purification of ascorbate oxidase from Cucurbita pepo medullosa (field pumpkin), with improved yields. The enzyme is biochemically characterized, including Michaelis-Menten parameters. Also, purification and characterization of the peroxidase from the same source is described, featuring an unusually high affinity for hydrogen peroxide(low-micromolar).

Keywords: ascorbate oxidase, peroxidase, field pumpkin, purification 		 
         
     
         
         
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