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STUDIA CHEMIA - Issue no. 1 / 2024 | |||||||
Article: |
GC-MS AND HPLC CHROMATOGRAPHIC PROFILE OF MAJORITY VOLATILE AND PHENOLIC COMPOUNDS OF SOME MEDICINAL PLANTS FROM ROMANIA. Authors: IRINA CIOTLĂUȘ , ANA BALEA, MARIA POJAR-FENEȘAN, FILIP MIUȚA, MIHAELA VLASSA. |
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Abstract: DOI: 10.24193/subbchem.2024.1.08 Published Online: 2024-03-30 Published Print: 2024-03-30 pp. 121-145 VIEW PDF FULL PDF This study reports the identification of volatile organic compounds (VOCs) and the phenolic composition for these medicinal plants: lemon balm (Melissa officinalis), lavender (Lavandula angustifolia), and elderflower (Sambucus nigra). The HS-SPME-GC-MS hyphenated technique was used to investigate the volatiles from the three plants in fresh and dried forms. The essential oils were obtained by hydrodistillation technique, followed by GC-MS analysis. Additionally, HPLC-UV/VIS detection was used to identify the phenolic compounds of these plants. The majority compounds identified in the fresh, dried and oil of lemon balm were Z-beta-ocimene, citronellal, citronellol, b-caryophyllene, (E)-citral, (Z)-citral and geraniol respectively. The aerial part of lavender contains mainly linalool, linalyl acetate, beta-myrcene, trans-beta-ocimene, lavandulyl acetate and caryophyllene. The most compounds identified in the fresh flowers of elderflower were linalool, cis-beta-ocimene, linalool oxide (II) pyran, cis-3-hexenyl isovalerate, while in the dry flowers the majority compounds were linalool oxide (II) pyran, cis-3-hexenyl isovalerate and hexenyl tiglate. The essential oil was rich in n-hexadecanoic acid, linoleic acid, and heneicosane. Most phenolic compounds identified in the analysed species were vanillic, sinapic, ferulic, and p-coumaric acids, while the predominant flavonoids were rutin, quercetin and epicatechin. The profile of VOCs represents an indicator in the valorisation of medicinal plants. Keywords: medicinal plants, volatile organic compounds (VOCs), phenolic compounds, HS-SPME-GC-MS, HPLC-UV/VIS |
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