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AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name. |
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STUDIA CHEMIA - Issue no. 3 / 2021 | |||||||
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SINGLE-MOLECULE DETECTION AND MANIPULATION WITH BIOLOGICAL NANOPORES. Authors: IRINA SCHIOPU, ALINA ASANDEI, LOREDANA MEREUTA, ISABELA DRAGOMIR, CEZARA BUCATARU, TUDOR LUCHIAN. |
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Abstract: DOI: 10.24193/subbchem.2021.3.09 Published Online: 2021-09-30 Published Print: 2021-09-30 pp. 161-174 VIEW PDF FULL PDF Single-molecule electrophysiology techniques using protein-based or solid-state nanopores as nanoreactors were proven incredibly useful as platforms for sensing and biophysical characterisation of biological molecules (e.g., peptides, proteins), DNA detection and sequencing in a label-free, low-cost, rapid and high signal-to-noise ratio manner. Herein we present a number of discoveries in this field, developed over the years in our laboratory, including: (i) the pH-mediated, fine-tuning of peptides passage through the α-hemolysin nanopore; (ii) increase of the capture rate and dwell times of polypeptides inside the nanopore, through engineering dipole-like polypeptides; (iii) the implication of a nanopore-AuNP (citrate anion-coated gold nanoparticles) platform to selectively detect nanomolar concentrations of target ssDNA. Keywords: single-molecule, peptides, electrophysiology, detection, sensing, nanopore, DNA, PNA |
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