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AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name. |
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STUDIA CHEMIA - Issue no. 2, Tom II / 2017 | |||||||
Article: |
A SENSITIVE ANALYTICAL (RP-HPLC-PDA, UV/VIS) METHOD FOR THE DETERMINATION OF NEWLY SYNTHESIZED N-ISONICOTINOYL-Nˊ-(3-FLUOROBENZAL)HYDRAZONE (SH2) IN AQUEOUS PHASE. Authors: ZVEZDELINA YANEVA, NEDYALKA GEORGIEVA. |
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Abstract: DOI: https://doi.org/10.24193/subbchem.2017.2.15 Published Online: 2017-06-20 Published Print: 2017-06-30 VIEW PDF: A SENSITIVE ANALYTICAL (RP-HPLC-PDA, UV/VIS) METHOD… The purpose of the present study was to develop a simple, rapid and reproducible analytical method for the determination of N-isonicotynoyl-Nˊ-(3-fluorobenzal)hydrazone (SH2) - a synthesized by us halogenated isoniazid derivative with high tuberculostatic activity, in aqueous phase, on the basis of RP-HPLC-PDA and UV/VIS spectrophotometric investigations. Despite of the high linearity (R2 0.9984) of the UV/VIS spectrophotometric method applied, the significantly higher LOQ and LOD values indicated its unsuitability for detection and quantification of low N-isonicotynoyl-Nˊ-(3-fluorobenzal)hydrazone concentrations (< 10 µg/mL). The proposed RP-HPLC-PDA method with mobile phase ACN/phosphate buffer (60:40, v/v) offered short retention time (3.1 min), high precision (RSD 3.50 %) and linearity (R2 0.9898). It characterized with satisfactory LOD (0.346 µg/mL) and LOQ (1.05 µg/mL) values and allowed the qualitative detection of SH2 E/Z-isomer. Keywords: N-isonicotynoyl-Nˊ-(3-fluorobenzal)hydrazone, RP-HPLC-PDA, UV/VIS, aqueous phase |
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