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AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name. |
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STUDIA BIOLOGIA - Issue no. 1 / 2012 | |||||||
Article: |
NEW PCR PRIMERS FOR PARTIAL YCF1 AMPLIFICATION IN ASTRAGALUS (FABACEAE): PROMISING SOURCE FOR GENUS-WIDE PHYLOGENIES. Authors: LÁSZLÓ BARTHA, GÁBOR SRAMKÓ, NICOLAE DRAGOŞ. |
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Abstract: New primer combinations are proposed for PCR amplification and sequencing of an approx. 1.5 kb long fragment from the 3’-end of Astragalus hypothetical chloroplast open reading frame 1 (ycf1). The efficiency of primers has been tested and confirmed in a wide variety of Old World taxa (including annuals, perennials with basifixed and medifixed hairs) and Oxytropis pilosa. The sequenced region showed elevated sequence variability and a consistent phylogenetic potential. Ycf1 has been traditionally treated cautiously because of the putative selective pressure acting on it but its popularity as a phylogenetic marker has started to increase recently (applied within angiosperms only in Orchideaceae and Lamiaceae so far). We, therefore, tested for the phylogenetic utility of ycf1 in Astragalus employing Maximum Parsimony analysis of the 19 Astragalus and one Oxytropis sequences generated in this study. The resulted tree topology reflected several previously revealed relationships based on ITS sequences of the nuclear ribosomal DNA region or affinities expected from morphology based taxonomic treatments. Taxa of sections Caprini, Dissitiflori and Incani formed monophylous entities, whereas the position of taxa belonging to sections Craccina and Hypoglottidei revealed the paraphyletic status of these sections. The resulted close relationship between A. cicer (sect. Hypoglottidei) and A. onobrychis (sect. Onobrychoidei) warrants further investigations on the re-circumscription of these sections. We propose using ycf1 as a phylogenetic marker in Astragalus that – especially in combination with other chloroplast markers – might be a useful tool for monophyly testing and sectional delimitation. Keywords: Astragalus, ycf1, chloroplast DNA phylogeny |
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