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    STUDIA PHYSICA - Issue no. 2 / 2010  
         
  Article:   METHOD VALIDATION FOR FREE AMINO ACIDS DETERMINATION IN BIOLOGICAL SAMPLES.

Authors:  ANDREEA IORDACHE, MONICA CULEA.
 
       
         
  Abstract:  A sensitive analytical method for free amino acids determination in biological samples was developed by isotopic dilution mass spectrometry. The method was validated. Deconvolution of the ion abundance ratios to yield tracer-to-tracee ratios for the isotopomers was done using Brauman’s least squares approach. Significant differences were observed between the amino acid levels measured in biological samples.

Keywords: amino acids, isotope dilution, gas chromatography-mass spectrometry. 

 
         
     
         
         
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