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    STUDIA PHYSICA - Issue no. 2 / 2005  
         
  Article:   ATR-FTIR INVESTIGATIONS OF SECONDARY STRUCTURE OF LYOPHILIZED BLOOD PLASMA IN PREGNANCY INDUCED HYPERTENSION.

Authors:  LAVINIA SABĂU, GHEORGHE DAMIAN.
 
       
         
  Abstract:  Pregnancy-induced hypertension (PIH) knowns by many clinical names as toxaemia, gestational proteinuric hypertension and preeclampsia is not a simply high blood pressure, but is a pregnancy condition which must be taken seriously as it can pose a serious risk to health of both mother and baby. This is certainly one of the characteristic signs of the condition, the others being protein in the urine and fluid retention. By biocheemical investigations, we found significantly higher values (p<0.001) of serum carbonyl content of proteins in patients with preeclampsia than in healthy pregnant women having the same gestational age. These results illustrate a great production of reactive oxygen species for pregnant women with preeclampsia. Many studies have shown that reactive oxygen species produced in excess modify the structure of proteins, lipids and nucleic acids. The changes in the secondary structure of blood plasma, we have investigated in the lyophilized blood plasma from 30 patients, healty and with different degree of diseases, by ATR-FTIR spectroscopy. In case of patients with preeclampsia the ATR-FTIR spectroscopy reveals a decrease in α-helix and β-sheet content and an increasing of aggregates content. The changes in the secondary structure of blood plasma observed by ATR-FTIR spectroscopy are in perfect correlation with the results obtained from the biochemical method of determination the damage on proteins due to oxidative stress.  
         
     
         
         
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