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    STUDIA PHYSICA - Issue no. 2 / 2002  
         
  Article:   INVESTIGATION OF MOLECULAR MOBILITY OF SPIN LABELS IN BSA.

Authors:  GHEORGHE DAMIAN. 		 
       
         
  Abstract:  EPR spectroscopy was used to study the interaction unsaturated spin label 3-carbamoyl-2,2,5,5-tetramethyl-3-pyrolin-1-yloxy (Tempyo) and bovine serum albumin molecules and bovine serum albumin (BSA). The EPR spectrum of the spin label in the low lyophilized state, exhibits two spectral parts: the major part has a line shape typical for slow isotropic rotational motion, and the minor part typical for faster isotropic rotational fluctuations. The minor parts have a rotational correlation time of 3-4•10-9 s, which must be regarded as fast rotation and indicate that these spin adducts are located in flexible nonpolar protein chains, probably at the surface of the protein and the major parts of the spectra have correlation times of 1.8-2.0•10-8 s, as result of strong interaction of spin label with polar proteins sites or located inside the globular protein. Quantification by integration of the spectrum yielded ~20 % of the spin label with fast motion and ~80 % with slow motion.  
         
     
         
         
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