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    STUDIA PHYSICA - Issue no. 1 / 2009  
         
  Article:   UV-VIS AND FLUORESCENCE STUDY ON PROTEIN ENRICHED SIMULATED BODY FLUID.

Authors:  E. LÁSZLOFFI, K. MAGYARI, VIORICA SIMON. 		 
       
         
  Abstract:  UV-vis and fluorescence analyses were carried out to investigate the spectroscopic changes induced by increasing concentration of bovine serum albumin in a solution of simulated body fluid, prepared according to Kokubo protocol. In this study are presented the results obtained from as prepared solutions. The linear increase of absorbance in UV-vis spectral region can be used to evaluate the protein concentration in solution. The fluorescence intensity dependence on BSA concentration has an inflection point at 1.41 mg/ml BSA in SBF, denoting a diminished fluorescence quantum yield for higher BSA concentration.

Keywords: Bovine serum albumin; simulated body fluid; fluorescence; UV-vis spectroscopy 		 
         
     
         
         
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