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Biological wastewater treatment is the most common technology for municipal wastewater purification, where different consortia of microorganisms aggregated in sludge flocks degrade organic matter in wastewater; activity and distribution of enzymes in flocks reflects microbial activities. A lab-scale sequencing batch reactor (SBR) was built and used for the treatment of synthetic wastewater to investigate the activity and distribution of hydrolytic enzymes (α-amylase, α-glycosidase and alkaline phosphatase) in sludge flocks. The enzymatic activity parameters of the enzymes were analyzed in function of chemical oxygen demand values of samples to determine the activity and distribution in the course of the whole process, aiming in the future to a better understanding of biological processes that occur during wastewater treatment.

Keywords: extracellular enzymes, enzyme activity, enzyme distribution, sequencing batch reactor, activated sludge flocs