AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: MIHAELA VLASSA, MIUȚA FILIP, VIRGINIA COMAN, VLAD PĂNESCU, CĂTĂLINA HERGHELEGIU, MIHAIL SIMION BELDEAN-GALEA.
DOI: 10.24193/subbchem.2022.4.18
Published Online: 2022-12-30
Published Print: 2022-12-30
pp. 273-285
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Abstract. This paper purpose was to develop a sensitive and selective method for the determination of glyphosate, and aminomethylphosphonic acid (AMPA) residues in water and glyphosate from soil samples. The method involves a derivatization step with 9-fluorenylmethylchloroformate in borate buffer of these compounds and liquid chromatography separation with fluorescence detection (HPLC-FLD). Separation of derivatized glyphosate and AMPA compounds was performed on an Agilent ZORBAX C18 reversed-phase column. The mobile phase consisted of a mixture of acetonitrile and 0.05 M KH2PO4 solution [30:70 v/v]. Limits of detection (LOD) was 0.28 µg L-1 for glyphosate, and 0.35 µg L-1 for AMPA, and limits of quantification (LOQ) was 0.84 µg L-1 for glyphosate and 1.05 µg L-1 for AMPA. The method has been validated for surface water and soil by recovery studies with samples spiked at 25 and 5 µg L-1. In water samples, the mean recoveries values ranged between 86.44 - 103.9% for glyphosate, and 71.27 - 99.08% for AMPA. The mean recoveries values for glyphosate ranged from 57 - 81.5% in soil samples. The developed method has been applied for determination of these compounds in water and soil samples from agricultural and rural areas of Roma community from some Transylvania Counties.
Keywords: Glyphosate, aminomethylphosphonic acid, water, soil, FMOC-Cl, RP-HPLC-FLD.
