The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary

The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name.

  
       
         
    STUDIA CHEMIA - Issue no. 2 / 2016  
         
  Article:   PRODUCTION OF CHEMICALS WITH GENETICALLY MODIFIED ESCHERICHIA COLI STRAINS FROM RENEWABLE RESOURCES.

Authors:  SZABOLCS LÁNYI. 		 
       
         
  Abstract:   The main aim of this study was to create mutant strains with lambda Red recombination system from an Escherichia coli strain which was isolated from animal faeces. E. coli Ter8/1 strain was genetically modified using lambda Red recombination system and two mutant strains were obtained, TerP01, in which the gene of pyruvate formate lyase was inactivated, and TerPL02, in which the genes of pyruvate formate lyase and lactate dehydrogenase were inactivated. The analysis of product formation in dual-phase fermentation and in minimal salts medium with three carbon sources was performed. The first mutant, TerP01, produced a large amount of lactic acid with small amount of byproduct formation. In the case of the second mutant, TerPL02, lactic acid production has been finished and succinic acid production increased significantly.

Keywords: Escherichia coli, lambda Red recombination system, fermentation, glycerin, glucose, xylose
 
         
     
         
         
      Back to previous page