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    STUDIA CHEMIA - Issue no. 2 / 2013  
         
  Article:   SITE DIRECTED SPIN LABELING OF HEMERYTHRIN AND HEMOGLOBIN .

Authors:  RADU SILAGHI-DUMITRESCU, GRIGORE DAMIAN.
 
       
         
  Abstract:   Site directed spin labeling in combination with electron paramagnetic resonance spectroscopy has become a very effective tool for studying the dynamics and structure of biomolecules in their native environment. This work presents the basics of site directed spin labeling and provides our results obtained in spin labeling hemerythrin and hemoglobin using methanethiosulfonate spin label. Best fit magnetic and libration parameters are obtained by simulation of EPR spectra.

Keywords: site-directed spin labeling, EPR, proteins, nitroxides
 
         
     
         
         
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