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    STUDIA CHEMIA - Issue no. 1-2 / 1999  
         
  Article:   BIOELECTRODE BASED ON TYROSINASE ENTRAPMENT IN ELECTROPOLYMERIZED MATRIX FOR AMPEROMETRIC DETECTION OF PHENOLIC COMPOUNDS.

Authors:  SARMIZA ELENA STANCA, IONEL CÄ‚TÄ‚LIN POPESCU, LIVIU ONICIU. 		 
       
         
  Abstract:  Based on simplicity and low enzyme denaturation, physical methods for enzyme immobilization were extensively used for biosensor construction. In this context, two “in situ” obtained polymer matrix, polyaniline and poly-amphiphilic pyrrole, were compared for tyrosinase immobilization on Pt electrodes, in order to construct bioelectrodes for phenol amperometric detection. Mixtures of tyrosinase and the corresponding monomer were electropolymerized, in the presence of a supporting electrolyte (0.1 M LiClO4), at 0.75 and 0.45 V vs. SCE for amphiphilic pyrrole derivative and for aniline, respectively. Steady state amperometric measurements, performed at -180 mV vs. SCE (aqueous buffer, KCl 0.1 M, phosphate tampon pH=7) and -50 mV vs. SCE (0.1 M C6H5CH2N(CH2)3Cl in CHCl3), were used to estimate the bioelectrodes electroanalytical parameters. It was established that the polypyrrole matrix has a higher efficiency for enzyme retention resulting in higher bioelectrode sensitivity both in aqueous or chloroform media.  
         
     
         
         
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