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    STUDIA CHEMIA - Issue no. 1 / 2013  
         
  Article:   THE ANALYSIS OF COUMARINS FROM SCOPOLIA CARNIOLICA JACQ. (SOLANACEAE) OF ROMANIAN SPONTANEOUS FLORA.

Authors:  LAURIAN VLASE.
 
       
         
  Abstract:  Coumarins from Scopolia carniolica Jacq. (Solanaceae), henbane bell, have been analysed. The qualitative analysis was performed by thin layer chromatography (TLC), scopoletin and two of its glycosides being emphasized. Scopoletin (free and total) was quantitatively determined by LC/MS techniques. The dynamics of coumarin accumulation was analysed in different vegetative organs harvested at 2-4 weeks interval during the vegetation period. The highest amount of free scopoletin was found in the underground organs before fruit maturation, after that period being identified in the aerial parts. The total scopoletin amount, determined after hydrolysis, was up to 70 times higher than the free scopoletin one, indicating that it is preferentially accumulated as glycosides, mostly in the rhizomes and roots.

Keywords: scopoletin, LC/MS, Scopolia carniolica
 
         
     
         
         
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