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    STUDIA CHEMIA - Issue no. 1 / 2013  
         
  Article:   EFFECTS OF A SINGLE DOSE OF ULTRAVIOLET B IRRADIATION ON OXIDANT/ANTIOXIDANT BALANCE IN THE EYE OF WISTAR RATS.

Authors:  VIORICA SIMON.
 
       
         
  Abstract:  Solar ultraviolet radiation (UV) is a major cause of ocular injury contributing to photokeratitis, cataract and pterygium development. The aim of the study was to investigate the oxidant/antioxidant status of Wistar rat eyes after exposure to various doses of UVB in correlation with morphological and structural changes. Five groups of 8 animals each, randomly divided, were treated as follows: group 1: control, no UVB irradiation; group 2: a single UVB exposure to a dose of 45 mJ/cm2; group 3: a single UVB exposure to 90 mJ/cm2; group 4: a single UVB exposure to 180 mJ/cm2; group 5: a single UVB exposure to 360 mJ/cm2. At 24 hrs after UVB irradiation the animals were anaesthetized and sacrificed by cervical dislocation. The rat eyes from 5 animals were extracted and used for biochemical determinations and from 3 animals were harvested and used for histopathological investigation. Our results demonstrated that a single UVB exposure at different doses disturbs the oxidant-antioxidant balance in the eye tissues by lipid peroxides generation, activation of CAT and SOD and adaptative increasing of GSH levels, particularly at high doses. Twenty hours following UVB irradiation the cornea showed significant lesions: inflammation, hemorrhage, superficial/deep ulcerous keratitis and epithelial exfoliation. Severity of injuries was dose-dependent. These data suggest that oxidative stress may be responsible for the corneal lesions induced by UVB irradiation.

Keywords: ultraviolet radiation, oxidative stress, eye, Wistar rats
 
         
     
         
         
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