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    STUDIA CHEMIA - Issue no. 1 / 2009  
         
  Article:   THE INFLUENCE OF SOLID SURFACE ON THE ADSORPTION OF ANTI-C1Q MONOCLONAL ANTIBODY AT ISOELECTRIC PH.

Authors:  IOAN BALEA, MARIA TOMOAIA-COTISEL, OSSI HOROVITZ, GHEORGHE TOMOAIA, AURORA MOCANU. 		 
       
         
  Abstract:   The effect of solid surface and ionic strength, in a buffer solution at isoelectric pH (5.5), on the surface immobilization, and adsorption process of a mouse monoclonal antibody, type IgG1, anti-C1q, is investigated by atomic force microscopy (AFM). The adsorption is conducted on glass, amino silanized glass, and positively charged modified glass. The AFM images present a monolayer adsorption with a dominant lying flat orientation of the molecules. This suggests that van der Waals interactions and possibly hydrogen bonds between antibody molecules and the solid substrate are the dominant interactions upon electrostatic forces, in conditions of rather low surface charge densities, high ionic strength of buffer solutions, and antibody molecules without a net charge.

Keywords: anti-C1q monoclonal antibody, adsorption, AFM, ionic strength, surface charge 		 
         
     
         
         
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