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    STUDIA CHEMIA - Issue no. 1 / 2004  
         
  Article:   THIRD GENERATION AMPEROMETRIC BIOSENSOR BASED ON MEDIATED ELECTRON TRANSFER BETWEEN ALCOHOL DEHYDROGENASE AND 1,1,2,2,3,3,4,6,7,8,9-UNDECACHLORO-1,2-DIHYDRO-PHENOTHIAZINE.

Authors:  DELIA M. GLIGOR, GRAZIELLA LIANA TURDEAN, LIANA MARIA MUREȘAN, IONEL CĂTĂLIN POPESCU. 		 
       
         
  Abstract:  A new amperometric biosensor for ethanol was obtained using a graphite electrode modified with 1,1,2,2,3,3,4,6,7,8,9-undecachloro-1,2-dihydro-phenothiazine as transducer, and alcohol dehydrogenase (ADH) cross-linked with glutaraldehyde, as NAD+/NADH dependent detecting enzyme. The ethanol amperometric detection is based on the oxidation of NADH generated in the enzymatic reaction between ethanol and NAD+ in the presence of ADH. The biosensor response increases linearly with ethanol concentration up to 2 M and the detection limit was 1.5 mM. The biosensor activity remains relatively stable for about 2 days.  
         
     
         
         
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