AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: RALUCA-ALINA MUSTAȚĂ, IMOLA MOLNAR, TUNDE-EVA DENES, ELENA RAKOSY-TICAN.
Potato, an excellent staple food, is one of the main targets for genetic improvement by combinatorial biotechnology. The aim of the present study was to combine gene transfer with in vitro stress selection to obtain plants resistant to both PVY and drought stress. Our hypothesis is that one resistance to one type of stress might determine a state of better response to a second stress factor. Marker free, transgenic potato lines were tested for resistance to drought under in vitro polyethylene glycol (PEG) induced stress selection. Callus cultures have been obtained from internodes of potato cultivars: Baltica and Désirée, of control and transgenic lines, respectively. The drought stress was simulated in vitro for three weeks on MS-T medium with 5% PEG 6000. The regenerated plants were selected in a second round on RMB5 medium with 5%, 10% and 15% PEG. At the end of each stage, the plants were evaluated for viability, root development and the regeneration of axillary buds. Two clones of cv. Baltica putatively tolerant to drought and resistant to PVY were obtained.
Keywords: drought, PEG, potato stress selection.
