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Community-acquired and hospital-acquired systemic staphylococcal infections are two major causes of mortality worldwide. A total of 125 staphylococcal isolates collected in Sibiu between July 2008 and January 2010 were used in this study. Twenty three of them were confirmed using the mecA/nucA duplex PCR technique; ten strains contains the nucA gene and were considered Staphylococcus aureus. The rest of 13 methicillin-resistant strains were tested using the API Staph commercial identification system (bioMérieux, France).

Keywords: API Staph, gene, PCR, Staphylococcus.