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    STUDIA BIOLOGIA - Issue no. 2 / 2008  
         
  Article:   PHYSIOLOGICAL STUDIES ON THE NITROGEN FIXING BACTERIA ISOLATED FROM MOUNTAINOUS SOILS.

Authors:  RAHELA CARPA, MIHAIL DRĂGAN-BULARDA. 		 
       
         
  Abstract:  Studies were carried out upon some nitrogen fixing bacteria strains isolated from five altitudinal vegetation zones of Parâng Mountains. These physiological analyses consisted of studying the influence of four different temperatures on the growth and development of Azotobacter strains isolated from mountainous soils. These strains were cultivated on mannitol medium and on sucrose medium at pH 7 and continous shaking to 150 rpm. The nitrogen fixing capacity of the strains originating from Parâng at the chosen temperatures was determined indirectly. Through these indirect methods the products resulted from nitrogen fixation were measured, namely extracellular proteins (by Lowry modified method) and produced ammonia secretion in culture medium (by Nessler method).

KEYWORDS: Azotobacter, physiological analysis, nitrogen fixation 		 
         
     
         
         
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