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    STUDIA BIOLOGIA - Issue no. 2 / 2003  
         
  Article:   MICROBIOTA UNOR APE MINERALE DIN ZONA LĂPUŞULUI (JUD. MARAMUREŞ).

Authors:  MIHAIL DRĂGAN-BULARDA, CLAUDIU IUŞAN.
 
       
         
  Abstract:  Microbiota of Some Mineral Waters from the Lpu Zone (Maramure County). The microbiota of mineral waters is closely related to the environmental, physical-chemical and hydro-geological conditions of the deposit. Our research is the first complex analysis of the mineral waters in the Lpu zone (Maramure county) from a microbiological point of view. We studied 9 springs of mineral water, at four places in the Lpu Valley (Stoiceni, Rogoz, Borcut and Mgureni). We determined the numbers of the following bacteria: heterotrophic aerobic, ammonifying, nitrite and nitrate-producing, denitrifying, sulphate-reducing, iron-reducing and proved the presence of the sulphur-oxidizing bacteria in the water samples taken aseptically in October 2002. We also determined four hygienic-bacteriological indicators: the mesophilic heterotrophic bacteria, the total coliform bacteria, the faecal coliform bacteria and the faecal enterococci , in the water samples collected aseptically in April 2003. Our data show that all the bacteria in the seven physiological groups were present in a low or very low number in the studied springs, the mineral waters being oligotrophic, containing a very low amount of organic substance. Some differences were observed between the mineral water springs based on their bacterial content. Occurrence of sulphur-oxidizing bacteria was demonstrated in water samples from all springs by using three elective media. On the basis of the average pH value modification and of the microscopic examination, it was established that these bacteria belong to the Thiobacillus genus. Mesophilic heterotrophic bacteria were found in all water samples but in a small number. The numbers recorded for the total coliform bacteria were low in all mineral water samples. Faecal coliform bacteria were present in 6 of the 9 springs (their number was small), but the faecal enterococci were missing in all analysed water samples. These data confirm a good hygienic-sanitary state of the springs and installations.  
         
     
         
         
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