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    STUDIA BIOLOGIA - Issue no. 2 / 2002  
         
  Article:   THE PRESENCE OF SULPHATE-REDUCING BACTERIA IN THE BOTTOM SEDIMENTS OF THE ROMANIAN BLACK SEA AREA.

Authors:  ELENA STOICA, MIHAIL DRÄ‚GAN-BULARDA, JAN KUEVER. 		 
       
         
  Abstract:  Seven strains (Et1, But1, But2, But3, Lac1, Isob1, Benz1) of Gram-negative, mesophilic, nonsporing sulphate-reducing bacteria were isolated from bottom sediments of the Romanian Black Sea coast. Analysis of partial 16S rDNA sequences, obtained from pure cultures of isolated strains by PCR, revealed that all strains belonged to the  - subclass of Proteobacteria. Three strains (But1, But3, Lac1) were morphologically and nutritionally similar. According to their 16S rDNA sequences, the isolates were affiliated with the following species: Desulfofrigus fragile (But1, But3, Lac1, 97.9–98% similarity), Desulfovibrio acrylicus (Et1, 98% similarity), Desulfobacterium autotrophicum (But2, 99% similarity), Desulfobacterium niacini (Isob1, 99% similarity). Strain Benz1 represents a new species of the genus Desulfobacula and had 96% sequence similarity to the previously described species. This is a first description of the diversity (community structure) of sulphate-reducing bacteria from the marine sediments of the Romanian Black Sea coast by molecular techniques.  
         
     
         
         
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