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    STUDIA BIOLOGIA - Issue no. 1 / 2019  
         
  Article:   REASSESSMENT OF THE TAXONOMIC POSITION OF THE GENERA HALOTERRIGENA AND NATRINEMA.

Authors:  HIROAKI MINEGISHI, YASUHIRO SHIMANE, MASAHIRO KAMEKURA, ANTONIO VENTOSA, TAKASHI ITOH.
 
       
         
  Abstract:   The genus Natrinema was created in 1998 to accommodate some misclassified halobacterial strains, while the genus Haloterrigena was established in the following year. Since then, it has been known that the two genera overlap to some extent in phylogenetic trees based on 16S rRNA gene sequences. The numbers of species of genera Haloterrigena and Natrinema increased to six and five, respectively by 2019. We suggested that the two genera might constitute a single genus based on studies of full-length gene sequences of the RNA polymerase subunit B'', as well as 16S rRNA gene sequences. Recently, Ding et al. (2017) and Rasooli et al. (2017) showed that species of the genera Natrinema (7 species) and Haloterrigena (10 species) formed a monophyletic group in 16S rRNA gene sequence trees generated using neighbour-joining and maximum likelihood algorithms. Reclassification of species presently belonging to the genus Haloterrigena as Natrinema species may be justified in the near future. In this study, the relatedness of 17 species belonging to the genera Haloterrigena and Natrinema was investigated through phylogenetic analysis (by the use of 16S rRNA and rpoB'' genes) and the assessment of the average nucleotide blast identity (ANI) and the percentage of conserved proteins (POCP). Results of ANI vs POCP and 16S rRNA gene vs POCP with 17 species in the genera Haloterrigena and Natrinema, confirmed that these strains are very likely to constitute a monophyletic group.

Keywords: 16S rRNA gene, ANI, Haloterrigena, Natrinema, POCP
 
         
     
         
         
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