STUDIA UNIVERSITATIS

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STUDIA BIOLOGIA - Issue no. 1 / 2018

Article:

PRELIMINARY DATA REGARDING GENETIC DIVERSITY OF SEVERAL ENDANGERED AND ENDEMIC DIANTHUS SPECIES FROM ROMANIA GENERATED BY RAPD MARKERS.

Authors: ANCA-LIVIA BUTIUC-KEUL, LILIANA JARDA, IRINA GOIA, IRINA HOLOBIUC, ANCA FARKAS, VICTORIA CRISTEA.

Abstract:
DOI: 10.24193/subbbiol.2018.1.05

Published Online: 2018-06-22
Published Print: 2018-06-29

pp. 59-72

VIEW PDF: PRELIMINARY DATA REGARDING GENETIC DIVERSITY …

Conservation of endangered and endemic species of Dianhtus from Romania, requires the investigation of genetic polymorphism in the populations. Preliminary data were obtained by molecular characterization using RAPD markers. DNA amplification with the 9 RAPD primers of the individuals belonging to different populations of D. callizonus, D. giganteus ssp. banaticus, D. glacialis ssp. gelidus, D. henteri, D. nardiformis, D. pratensis ssp. racovitzae, D. spiculifolius and D. tenuifolius revealed low level of polymorphism within and between populations. Several polymorphic RAPD markers were identified being useful for investigation of genetic diversity. Out the 9 primers studied by us, only the primer OPB-07 ensured amplification in all species and primers OPA-13, OPE-04 and 1225 showed positive results in most of the species. The primers 4A-26 and 4A-27 ensured amplification only in D. spiculifolius and the primers 4A-23 and OPM-18 gave no results in none of the species.

Keywords: Dianthus, endemic/endangered species, genetic polymorphism, RAPD markers.

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	The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary The summary of the selected article appears at the bottom of the page. In order to get back to the contents of the issue this article belongs to you have to access the link from the title. In order to see all the articles of the archive which have as author/co-author one of the authors mentioned below, you have to access the link from the author's name.


	STUDIA BIOLOGIA - Issue no. 1 / 2018

	Article:	PRELIMINARY DATA REGARDING GENETIC DIVERSITY OF SEVERAL ENDANGERED AND ENDEMIC DIANTHUS SPECIES FROM ROMANIA GENERATED BY RAPD MARKERS. Authors: ANCA-LIVIA BUTIUC-KEUL, LILIANA JARDA, IRINA GOIA, IRINA HOLOBIUC, ANCA FARKAS, VICTORIA CRISTEA.


	Abstract: DOI: 10.24193/subbbiol.2018.1.05 Published Online: 2018-06-22 Published Print: 2018-06-29 pp. 59-72 VIEW PDF: PRELIMINARY DATA REGARDING GENETIC DIVERSITY … Conservation of endangered and endemic species of Dianhtus from Romania, requires the investigation of genetic polymorphism in the populations. Preliminary data were obtained by molecular characterization using RAPD markers. DNA amplification with the 9 RAPD primers of the individuals belonging to different populations of D. callizonus, D. giganteus ssp. banaticus, D. glacialis ssp. gelidus, D. henteri, D. nardiformis, D. pratensis ssp. racovitzae, D. spiculifolius and D. tenuifolius revealed low level of polymorphism within and between populations. Several polymorphic RAPD markers were identified being useful for investigation of genetic diversity. Out the 9 primers studied by us, only the primer OPB-07 ensured amplification in all species and primers OPA-13, OPE-04 and 1225 showed positive results in most of the species. The primers 4A-26 and 4A-27 ensured amplification only in D. spiculifolius and the primers 4A-23 and OPM-18 gave no results in none of the species. Keywords: Dianthus, endemic/endangered species, genetic polymorphism, RAPD markers.




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