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    STUDIA BIOLOGIA - Issue no. 1 / 2014  
         
  Article:   IN VITRO CYTOGENETIC STUDY OF THE MITOTIC DIVISION IN BASIL (OCIMUM BASILICUM L.) PLANTS.

Authors:  DIANA-ELENA MAFTEI.
 
       
         
  Abstract:  VIEW PDF: IN VITRO CYTOGENETIC STUDY OF THE MITOTIC DIVISION IN BASIL (OCIMUM BASILICUM L.) PLANTS

The cytogenetic studies on the in vitro - derived plants of Ocimum basilicum L. (var. Greek basil) aimed to evince if and to what extent this type of conventional culture altered the mitotic cell division. There were some differences regarding the mitotic index, the distribution of cells during each phase of mitosis, and also regarding the percentage of abnormal ana-telophases. The shoots obtained on a culture medium supplemented with indole butyric acid (IB2 medium variant) displayed the highest mitotic index (33.09), compared to the control (16.14) and to the other studied variants. The lowest mitotic index (7.39) was registered on the A2 variant, enriched with indole acetic acid. Regarding the cell distribution on mitotic phases, the highest percentage was registered by prophases, followed by telophases, metaphases, and anaphases (in all the analyzed variants, including the control). 

Keywords: basil, cytogenetic analysis, mitotic index
 
         
     
         
         
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