AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: ADORJÁN CRISTEA, ADRIAN-ŞTEFAN ANDREI, ANDREEA BARICZ, VASILE MUNTEAN, HORIA L. BANCIU.
Ursu Lake is a large saline, meromictic and heliothermal lake located in the eastern part of the Transylvanian Basin (Sovata, Mureș County, Romania). The investigated lake is characterized by a strong vertical stratification of physical and chemical parameters that indicate a corresponding stratification of biodiversity. Using BIOLOG EcoplateTM method we were able to describe the community-level physiological profile of the microbial population inhabiting the moderately saline epilimnion (5-6% salinity) of Ursu Lake. The metabolization of 31 different carbon sources was monitored in the water samples collected at 0.5 m depth from two different seasons: October 2013 and March 2014. Physico-chemical parameters (temperature, salinity, pH, oxidation-reduction potential, and dissolved oxygen) were measured along with the estimation of the total chlorophylls, carotenoids, and prokaryotic cell count. The results revealed a higher rate of C substrate consumption in the water sample collected in spring compared to that found in the autumn sample. This finding is paralleled by the differences observed in some of the chemical parameters (salinity, dissolved oxygen) between the seasons suggesting a time-based modification of the microbial activity. Alpha-cyclodextrin, glycogen, D-cellobiose, D-mannitol, and N-acetyl-D-glucosamine were the fastest metabolized C sources in both seasons. This is the first report of using BIOLOG EcoplateTM approach in profiling the microbial activity in a Romanian deep, meromictic and heliothermal salt lake and one of the very few attempts reported to use the BIOLOG system for the characterization of microbial communities in hypersaline ecosystems.
Keywords: BIOLOG EcoplateTM , community-level physiological
