AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Authors: ABDELMOUMEN TAOUTAOU, TÜNDE-ÉVA DÉNES, CONSTANTIN BOTEZ, ELENA RAKOSY-TICAN.
Potato, after cereals, is the most important crop with a production of more than 373 MT and 368 MT in 2011 and 2012, respectively. Late blight caused by the oomycete Phytophthora infestans is the most devastating disease of potato. Due to the concerns of consumers about possible adverse effects of GMOs and pathogen resistance to fungicides, resistance breeding is an optimal alternative. Conventional resistance breeding of potato against P. infestans was based on introgression of resistance (R) genes from the wild species S. demissum. However, this kind of resistance, based on S. demissum R genes, was easily overcome by the pathogen. Since genetic manipulation by gene transfer is still not accepted by the public and single gene resistance is short lived, somatic hybridization is a good option to overcome the sexual incompatibility. There are 5 families of R genes. All the R genes against P. infestans are members of the Nucleotie Binding Site-Leucine Rich Repeat (NBS-LRR) family. NBS domain is a highly conserved domain in the resistance genes toward pathogens. Primers constructed based on NBS domain sequence tag target the R genes and their homologues. In this study we used NBS primers to reveal the polymorphism between the somatic hybrids of potato in comparison with parental lines. Somatic hybrids between potato cultivars Delikat and Rasant with S. bulbocastanum, their backcross progenies (BC1) and parental lines, which carry two known resistance genes Rpi-blb1 and Rpi-blb3, one of those genes or none of them have been comparatively analyzed. The polymorphism of NBS domain will be also evaluated in relation with late blight resistance assays done with detached leaf assay and in the field. Acknowledgements. The authors are grateful to the project CNCS PNII-ID-PCE-2011-3-0586 for funding of this research and to the Life Science Institute of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca for technical support.
