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    STUDIA BIOLOGIA - Issue no. 1 / 2013  
         
  Article:   ORAL PRESENTATION ABSTRACTS: ROOT ARCHITECTURE OF BRACHYPODIUM – ROOT ARCHITECTURE OF BRACHYPODIUM – MORPHOLOGICAL, MOLECULAR AND GENETIC APPROACHES.

Authors:  MÁRIA SZÉCSÉNYI, ZOLTÁN ZOMBORI, MAGDOLNA GOMBOS, GYÖRGYI SÁNDOR, JÁNOS GYÖRGYEY.
 
       
         
  Abstract:  VIEW PDF: ORAL PRESENTATION ABSTRACTS

Root is the main plant organ that is responsible for water and nutrient uptake. Hence, its architecture determines strongly how it can fulfill the above role. On the other hand, the characteristics of root architecture are affected significantly by the underground environment, such as water and nutrient availability in the soil. In our group, characterization of various Brachypodium distachyon (L.) P. Beauv. ecotypes grown in pots or rhizotrons under either well-watered or reduced watering conditions is conducted. Comparison of plants grown in pots focuses mainly on the length and thickness of primary and nodal roots; however their photosynthetic and other shoot parameters are measured, too. Thus, the evaluation of different groups involving distinct ecotypes with similar shoot or root parameters can inform us about the importance of the presence/absence, thickness or length of different root types as well as their correlation with shoot parameters under optimal and adverse circumstances. Moreover, growing plants in rhizotrons allow us to follow the daily growth rate of primary, nodal and even lateral roots. Hence, the ecotypes can be distinguished on the basis of their root morphological plasticity in response to water-deficit stress. The plant-specific LBD (Lateral Organ Boundaries Domain) gene family is essential in the regulation of plant lateral organ development. Several LBD genes are related to almost all aspects of plant development, including embryo, root, leaf, and inflorescence development. Our group studies the transcript pattern of various LBD genes in different plant parts, selecting especially the root-specific ones that are probably involved in the lateral root formation in Brachypodium. Reverse genetic approaches of the above-selected LBD genes can inform us about their spatial and temporal expression. Therefore, transformants containing constructs with self-promoter and reporter gene together with transformants with ectopic over-expression of the selected LBD genes are being analyzed. Silencing of the selected LBD genes is also planned in the future.
 
         
     
         
         
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