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The STUDIA UNIVERSITATIS BABEŞ-BOLYAI issue article summary
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Abstract: The dialkyl - or alkyl/aryl esters of 1,2-benzenedicarboxylic acid, commonly known as phthalates, are high-production volume synthetic chemicals and ubiquitous environmental contaminants because of their use in plastics and other common consumer products. Globally there is significant concern for the ubiquitous presence of phthalates and their potential for causing adverse health effects in humans. Phthalates have possible teratogenic and carcinogenic effects, DEHP being included in Class B2 (probable human carcinogens). They are also suspected endocrine disruptors and an association between phthalate exposure and abnormal reproductive development has been suggested. In order to evaluate human exposure it is necessary to analyze the phthalates from environment, but also the phthalates metabolites from urine and serum. In this article it is presented a method for evaluate the concentration of phthalates metabolites in urine by enzymatic incubation and HPLC-MS/MS determination. Urine sample is conditioned with ammonium acetate solution. β –glucuronidase solution is added, the sample are sealed and incubated overnight at 37oC. The SPE cartridges pretreated with phosphate buffer and acetonitrile and are sample is cleaned up over them with formic acid and water. Phthalate metabolites are eluting with acetonitrile and ethyl acetate. The sample is concentrate near dryness under nitrogen stream and analyzed on a C18 column by HPLC-MS/MS. Linearity was controlled in the range between 0,5 -250 µg/l and the detection limit between 0,2 µg/l for most of the compounds and 1 µg/l for mono-(2-ethylhexyl)phthalate. Hereinafter are presented and discussed the characteristics of the method.
Key words: Phthalates metabolites, HPLC-MS/MS, Urine |
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