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    STUDIA PHYSICA - Ediţia nr.1 din 2001  
         
  Articol:   CARBON- 13 CONCENTRATIONS IN HUMAN BREATH MEASURED BY MASS SPECTROMETRY.

Autori:  STELA CUNA, O. COZAR, GABRIELA BALAS.
 
       
         
  Rezumat:  We have developed a specific and sensitiv test for detection of Helicobacter pylori that has been associated with gastrits, ulcers and some forms of gastric cancer. The test uses the ability of the urease (an enzyme produced by Helicobacter pylori) to break urea labeled with 13C to carbon dioxide and ammonia. The carbon dioxide (13CO2) is release first into the blood and very rapidly into the breath. 13CO2 is collected, purified and analysed by isotope ratio mass spectrometry. An increase in 13CO2 in the breath confirms that the patient is infected with Helicobacter pylori. This test has performed to a patient suspected to be infected with Helicobacter pylori. We used 75 mg 13C-urea and we have collected and analysed CO2 respiratory before the administration of 13C-urea and after the administration of 13C-urea at 20, 40 and 60 min. The results showed an increase up to 60 % of 13CO2 in the breath, it means an intensive activity of the urease, namely the patient has been infected with Helicobacter pylori. The validity of the results has been performed by endoscopy.  
         
     
         
         
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