Rezumat articol ediţie STUDIA UNIVERSITATIS BABEŞ-BOLYAI

În partea de jos este prezentat rezumatul articolului selectat. Pentru revenire la cuprinsul ediţiei din care face parte acest articol, se accesează linkul din titlu. Pentru vizualizarea tuturor articolelor din arhivă la care este autor/coautor unul din autorii de mai jos, se accesează linkul din numele autorului.

A simple and sensitive liquid chromatography coupled with tandem mass spectrometry (LC/MS3) method for the quantification of memantine in pig plasma was developed and validated. The separation was performed on a Zorbax SB-C18 column under isocratic conditions using a mobile phase of 55:45 (v/v) methanol and 0.1% (v/v) formic acid in water at 45 ºC with a flow rate of 1 mL/min. The detection of memantine was performed in multiple reaction monitoring mode using an ion trap mass spectrometer with electrospray positive ionisation, operating in MS³ mode. The pig plasma samples (0.2 mL) were deproteinized with 6% perchloric acid in water and aliquots of 10 μL from supernatants obtained after centrifugation were directly injected into the chromatographic system. The method shows a good linearity (r > 0.995), precision (CV < 12.2%) and accuracy (bias < 12.1%) over the range of 4.86-486 ng/mL plasma. The lower limit of quantification (LLOQ) was 4.86 ng/mL and the recovery was between 92.5-100.8%. The developed and validated method is simple, rapid and specific for the determination of memantine in pig plasma and was successfully applied to a pharmacokinetic study of intravenously administered memantine in pigs.

Keywords: memantine, LC/MS³, human plasma, pig plasma