AMBIENTUM BIOETHICA BIOLOGIA CHEMIA DIGITALIA DRAMATICA EDUCATIO ARTIS GYMNAST. ENGINEERING EPHEMERIDES EUROPAEA GEOGRAPHIA GEOLOGIA HISTORIA HISTORIA ARTIUM INFORMATICA IURISPRUDENTIA MATHEMATICA MUSICA NEGOTIA OECONOMICA PHILOLOGIA PHILOSOPHIA PHYSICA POLITICA PSYCHOLOGIA-PAEDAGOGIA SOCIOLOGIA THEOLOGIA CATHOLICA THEOLOGIA CATHOLICA LATIN THEOLOGIA GR.-CATH. VARAD THEOLOGIA ORTHODOXA THEOLOGIA REF. TRANSYLVAN
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Autori: IRINA SCHIOPU, ALINA ASANDEI, LOREDANA MEREUTA, ISABELA DRAGOMIR, CEZARA BUCATARU, TUDOR LUCHIAN.
DOI: 10.24193/subbchem.2021.3.09
Published Online: 2021-09-30
Published Print: 2021-09-30
pp. 161-174
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Single-molecule electrophysiology techniques using protein-based or solid-state nanopores as nanoreactors were proven incredibly useful as platforms for sensing and biophysical characterisation of biological molecules (e.g., peptides, proteins), DNA detection and sequencing in a label-free, low-cost, rapid and high signal-to-noise ratio manner. Herein we present a number of discoveries in this field, developed over the years in our laboratory, including: (i) the pH-mediated, fine-tuning of peptides passage through the α-hemolysin nanopore; (ii) increase of the capture rate and dwell times of polypeptides inside the nanopore, through engineering dipole-like polypeptides; (iii) the implication of a nanopore-AuNP (citrate anion-coated gold nanoparticles) platform to selectively detect nanomolar concentrations of target ssDNA.
Keywords: single-molecule, peptides, electrophysiology, detection, sensing, nanopore, DNA, PNA
