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    STUDIA CHEMIA - Ediţia nr.2 din 2016  
         
  Articol:   EXPRESSION AND PURIFICATION OF RECOMBINANT PHENYLALANINE 2,3-AMINOMUTASE FROM PANTOEA AGGLOMERANS.

Autori:  ANDREA VARGA, LÁSZLÓ CSABA BENCZE, LÁSZLÓ POPPE, CSABA PAIZS. 		 
       
         
  Rezumat:   In the present study, the gene of phenylalanine 2,3-aminomutase from Pantoea agglomerans (PaPAM) was cloned into pET-19b vector and used for its expression in competent Escherichia coli cells. The recombinant plasmid, PaPAM-pET-19b, was transformed into competent E. coli strain BL21(DE3)pLysS cells. Overnight culture of the transformed bacteria was induced by the addition of isopropylthio-β-D-galactoside (IPTG) to the final concentrations of 0.1, 0.5 and 1 mM. Also, the effects of different temperatures (18, 25 and 30°C) and the incubation time of PaPAM were examined. The fermentation process was scaled up to 10 L fermentor. Affinity purification conditions were analyzed by SDS-PAGE. The Tm and the activity of the purified enzyme was also investigated.

Keywords: phenylalanine 2,3-aminomutase, Pantoea agglomerans, optimization, protein expression
 
         
     
         
         
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