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    STUDIA CHEMIA - Ediţia nr.2 din 2013  
         
  Articol:   CHARACTERIZATION OF BUFFALO MILK FAT GLOBULES USING THE CONFOCAL LASER SCANNING MICROSCOPY.

Autori:  ROMOLICA MIHAIU, MARIAN MIHAIU.
 
       
         
  Rezumat:   Milk fat globules are biologically essential components due to their functional and health properties. Milk fat composition has been thoroughly studied in cow milk but still it remains unclear in buffalo milk. We have used confocal laser scanning microscopy (CLSM) to investigate the structure of the fat globules in cow and buffalo milk, using two types of fluorescently-labelled dyes for phospholipid and triglyceride constituents. Using this technique, we have observed heterogeneities in the distribution of these lipids both in the membrane as on the surface relating to the specie and size of the globules. The statistical analysis has shown that there are significant differences (p<0.05) among the average fluorescence intensity (13.68±9.98 AU/µm2) found at buffalo milk fat globules in comparison to cow ones (16.88 ± 4.3 AU/µm2). The statistical comparison of the phospholipids quantification values in both species revealed the fact that there are no significant differences (p>0.05), the average found at cow milk being 31.07 AU/µm2 and at buffalo 34.85 AU/µm2. The use of specific dyes may be essential in the evaluation of the unsaturated lipid milk fraction, the buffalo milk fat globules being easily differentiated from cow milk by OilRed quantification. 

Keywords: milk, buffalo, confocal laser scanning microscopy, fluorescence, dye
 
         
     
         
         
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